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Transferase, enzymes involved in protection against oxidative damage. Desiccation stress of nematodes caused significant up-regulation of transcripts encoding these genes [52,53]. Functional classification based on gene ontology Gene Ontology (GO) has been used widely to predict gene function and classification [54]. BLAST2GO [55,56], a universal, web-based annotation tool was used to assign GO terms for the A. avenae cluster sequences, extracting them from each BLAST hit against Swiss-Prot obtained by mapping to extant annotation associations. 1222 sequences out of 2,700 did not retrieve any BLAST results within the set E-value threshold (Capmatinib like protein ATP synthase alpha and beta subunits ENOL-1, enolase Kinase, protein-id: AAB37022.2 ACT-4, actin 4 ELF-4, elongation factor 1-alpha UBQ-1, ubiquitin like protein UNC-87, calponin Aspartate aminotransferase AAK2, protein kinase ABCF-2, ABC transporter Cytochrome c oxidase subunit 1 Protein-id:CAF31483.1 ATP-2, ATP synthase beta subunit GLN-3, glutamine synthetaseE-value 0 0 0 0 0 0 1e - 178 1e - 177 1e - 166 1e - 160 1e - 160 1e - 153 1e - 152 1e - 151 1e -Page 6 of(page number not for citation purposes)BMC Genomics 2009, 10:http://www.biomedcentral.com/1471-2164/10/sequences were successfully annotated and mapped to one or more of the three organizing principles of GO: biological process, molecular function and cellular component. The matches obtained from this analysis are summarized in Figs 4A-4C. 1,003 of the A. avenae cluster sequences generated matches in the "molecular function" class, 933 in the "biological process" class and 924 in the "cellular component" class. Within the "biological process" class the "regulation of biological process (GO:0050789)", "biosynthetic process (GO:0009058)" and "transport (GO:0006810)" categories were the most represented followed by "response to stimulus (GO:0050896) ", based(A)on the annotation assigned by BLAST2GO (Fig. 4A). Within the "molecular function" class the "protein binding (GO:0005515)" term is the most represented followed by "nucleotide binding (GO:0000166)" and "transferase activity (GO:0016740)" (Fig. 4B). Many cluster sequences encoding ribosomal proteins as well as highly expressed genes coding for structural molecules (such as actin) and regulatory molecules (such as transcription factors) are assigned to the "protein binding" term. Since those clusters are abundantly present in the dataset, this may cause overrepresentation of the "protein binding" term. Within the "cellular component" class the "mitochondrion (GO:0005739)" is the most highly represented (Fig. 4C). A complete listing of GO mappings(B)(C)Figure 4 Summary of the Gene Ontology annotation as assigned by PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/7500280 BLAST2GO Summary of the Gene Ontology annotation as assigned by BLAST2GO. (A) Most represent.