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Stitute for Medicine of Nanjing Command, Nanjing, China [41] [41]Hua Dong Research Institute for Medicine of Nanjing Command, Nanjing, China [41] Veterinary Practice Diessen, The Netherlands Veterinary Practice Diessen, The Netherlands Veterinary Practice Diessen, The Netherlands Veterinary Practice Diessen, The Netherlands Laboratory collection [9] Laboratory collection [9] Laboratory collection [9] Laboratory collection [9]Unknown Laboratory collection [9] Lung, pneumonia Stiftung Tier tzliche Hochschule Hannover, Hannover, Germany [25] Lung, pneumonia Stiftung Tier tzliche Celecoxib Hochschule Hannover, Hannover, Germany [25]de Greeff et al. BMC Microbiology 2011, 11:161 http://www.biomedcentral.com/1471-2180/11/Page 4 ofTable 1 Characteristics of bacterial strains used in this study (Continued)8074R7 7997 8067 8017 7709 C132 5973 22083R9 7998 8186 OV640 2840 Escherichia coli TG1 7 9 9 9 9 9 9 9 9 9 UT PA NA ND AV AV AV ND ND AV ND ND ND ND ND NA 25 16 136 136 16 16 137 82 16 138 139 134 NA * * * * * + * * NA * NA + + + + + + + NA Unknown Organs Unknown CNS Bacteraemia Brain/septicemia CNS Unknown Joint Tonsil Unknown Unknown NA Laboratory collection CVI Laboratory collection [9] Laboratory collection [9] Laboratory collection [9] Laboratory collection [9] Laboratory collection [9] Laboratory collection [9] Laboratory collection CVI Laboratory collection [9] Laboratory collection [9] Veterinary Practice Diessen, The Netherlands Laboratory collection Laboratory collectionRx reference strain of serotype x; UT untypable; PA, poly-agglutination; NA, not applicable; HV, highly virulent; V, virulent; WV, weakly virulent; AV, avirulent; ND, not determined; MRP, muramidase released protein; EF, extracellular factor; SLY, suilysin; +, protein expressed; -, PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/6833145 protein not expressed; *, higher MW protein expressed; s, smaller MW protein expressed; +/-, protein weakly expressed; CNS, central nervous system; TSLS, toxic shock-like syndrome * virulence as determined in an experimental infection in pigs # phenotype, protein expressioncolumn purified using Illustra Cyscribe GFX purification kit (GE Healthcare, Uppsala, Sweden) according to instructions of the manufacturer. Differential DNA presence was determined by two-colour fluorescent hybridizations of the corresponding genomic DNAs on the 8 ?15 k S. suis oligo array. Genomic DNA of each strain was cohybridized once with the reference strain P1/7, that was always labeled with Cy3. The test strain was consequently labeled with Cy5. Labeling of DNA (2,5 g) was done using the Bioprime Array CGH Genomic Labeling System (Invitrogen) with slight modifications as described by Molenaar et al., 2005 [29]. Labeling efficiency was measured using the Nanodrop (ThermoScientific, Wilmington, USA). Constant amounts of label (25 pmol each) were hybridized to the oligoarray in hybridization buffer of the In situ hybridization kit Plus (Agilent Technologies) following instructions of the manufacturer. During hybridization, slides were incubated for 17 h at 65 under rotation. Slides were washed for 10 min in 6 ?SSC/0.05 TritonX102 at room temperature, followed by 5 min in 0.1 ?SSC/0.05 Triton-X102 at 4 . Slides were dried using pressured air and scanned in a GenePix 4200AL scanner (Molecular Devices, Sunnyvale, USA). Scans were analyzed using GenePix software (Molecular Devices). Local background values were subtracted from the intensity of each spot. Data were normalized using S-Lowess [30] at the webtool accessible from h.